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KMID : 0357920010350040330
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Korean Journal of Pathology
2001 Volume.35 No. 4 p.330 ~ p.337
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Loss ofHeterozygosity on Chromosome 9p21, 17p13 and 3p in Human Astrocytic Tumor
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Lee Yeon-Soo
Kang Chang-Suk
Dong Seung-Myung
Lee Jung-Yong
Shim Sang-In
Kim Byung-Kee
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Abstract
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Background: The purpose of this study was to determine the genetic abnormalities of chromosomes gp21, 17p13.1, 3p25 and 3p14p.2 in the development and progression of astrocytic tumors.
Methode: We performed loss of heterozygosity （LOH）analysis in 41 astrocytic tumors, including 20 astrocytomas, 11 anaplastic astrocytomas and 10 glioblastomas, and correlate the results of LOH at different histopathologic grades. LOH was determined by multiplex polymerase chain reaction analysis of the DNA, which was extracted by microdessection.
Results: LOH of gp21 ws found in 55.6％ of astrocytomas, 54.6％ of anaplastic astrocytomas and 100.0％ of glioblastomas. LOH of 17p13.1 was found in 21.4％ of astrocytomas, 28.6％ of anaplastic astrocytomas, and 66.7％ of glioblastomas. LOH. of 3p25 was found in 37.5％ of astrocytomas, 16.7％ of anaplastic astrocytomas, and 14.3％ of glioblastomas. LOH of 3p14.2 was found in 16.7％ of astrocytomas, 40.0％ of anaplstic astrocytomas, and 42.9％ of glioblastomas. LOH on chromosome gp21 and 17p13.1 was closely related with the histopathologic grades.
Conclusions: Theses results may suggest that LOH of gp21, 17p13.1, 3p25 and 3p14.2 involves an early event of astrocytoma development and accumulates during progression. LOH of 3p25 may be involved in the tumorigenesis of astrocytoma. Ldentification of these LOH may illuminate the stepwise pathogenesis of astrocytic tumors and predict the possibility of malignant transformation.
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KEYWORD
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Astrocytoma, Loss of heterozygosity, 9p21, 17p13, 3p
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